期刊论文

Effect of Catechin on the Gene Expression of Cytokines and Phosphorylation Level of AMPK/SIRT1 in Macrophage and Adipocytes Co-cultured System

中文

中国食品学报

1009-7848

2021

21

04

64-70

10.16429/j.1009-7848.2021.04.008

山东省农业重大应用技术创新项目（SD2019ZZ016）； 湖南省教育厅专项（19A236）； 山东省泰山学者人才工程项目（tshw20120747）；

本校为第一机构

基于巨噬-脂肪细胞共培养体系研究儿茶素对细胞炎性因子、炎性酶基因表达及AMPK、SIRT1磷酸化水平的影响,初步阐明儿茶素的抗炎作用机制。儿茶素的添加质量浓度为0,50,100μg/mL,采用定量PCR方法检测细胞炎性因子及炎性酶基因的表达水平,采用Western blotting方法检测AMPK及SIRT1的磷酸化水平。结果表明,各处理组中,儿茶素明显抑制促炎性因子（IL-1、IL-6和IL-12）及炎性酶（iNOS和COX-2）基因的表达,促进抗炎性因子（IL-4和IL-10）基因的表达,并且提高AMPK和SIRT1的磷酸化水平,AMPK和SIRT1两者之间呈协同作用。共培养体系中巨噬细胞TNF-α的分泌可诱导脂肪细胞的分化与成熟,儿茶素通过AMPK/SIR...

Based on the macrophage and adipocytes co-cultured system,effect of catechins on the gene expression of cytokines and inflammatory enzymes,the phosphorylation levels of AMPK and SIRT1 was studied,and to preliminarily research the anti-inflammatory mechanism of catechin.Catechin with the mass concentrations of 0,50,100 μg/mL were added to the four treatments,respectively.The gene expression of cytokines and inflammatory enzymes,phosphorylation levels of AMPK and SIRT1 in adipocytes were determined by quantitative RT-PCR and Western blotting,res...