期刊论文

中文

中国油料作物学报

1007-9084

2008

30

3

361-365

10.3321/j.issn:1007-9084.2008.03.019

国家948项目“油菜特异资源和育种新技术的引进、改良及创新研究”（2006-Q04）；

本校为第一机构

利用~(60)Co-γ射线辐照的湘油15油菜种子，以Basta溶液为筛选压在油菜无性系中筛选出了抗除草剂突变体，随后通过打孔叶片抗性鉴定、除草剂喷洒鉴定等方法确定抗性。最后利用SRAP分子标记方法进行鉴定，从而为突变体的真实性提供了分子水平上的证据。

Mutagenic treatment was made to seeds of Xiangyou 15 with 60Co - γ/ray. As the result of stepwise positive selection with Basta, mutants were obtained with higher tolerance to herbicide. The herbicide - resistance ability were identified and determined after leaves punched and applied Basta directly. Genetic differences were detected by SRAP ma...