The browning reaction during the flue-curing process of tobacco (Nicotiana tabacum L.) dramatically affects leaf quality and value, leading to significant economic losses. However, the molecular regulatory mode of the browning in flue-cured tobacco is still unclear. The two tobacco cultivars, Dabaijin and Yunyan87, with browning differences in upper, middle, and lower leaves during the flue-curing process, were characterized through transcriptomic and metabolomic analyses. The results showed that the significant browning in Dabaijin was mainly due to the preferential accumulation of products o...