期刊论文

中文

中国病原生物学杂志

1673-5234

2022

17

2

186-190

湖南省重点领域研发计划(No.2019NK2181) 湖南农业大学“双一流”建设项目(No.SYL201802016)。

本校为第一机构

目的 对褐黄血蜱组织蛋白酶B（cathepsin L-like cysteine proteinase B,cathepsin L-lcp B）基因进行克隆及表达分析。方法 基于褐黄血蜱唾液腺转录组文库中的Contig731序列设计引物，RACE法扩增cathepsin L-lcp BcDNA（Hf-CLlCPb）全长，qPCR法分析Hf-CLlCPb在不同吸血状态下雌成蜱不同组织的表达水平。结果 Hf-CLlCPb全长1 547 bp, ORF框长1 005 bp,编码355个氨基酸；该基因在半饱血、饱血褐黄血蜱（♀）中的唾液腺表达量最高，卵巢、中肠、体壳次之；Hf-CLlCPb在半饱血褐黄血蜱（♀）中的表达量高于饱血褐黄血蜱（♀）。结论 成功克隆出褐黄血蜱Hf-CLlCPb基因全长，qPCR检测Hf-CLlCPb基因在...

Objective To clone and analyze the expression of Haemaphysalis flavacathepsin L-likecysteine proteinase B gene.Methods Primers were designed based on Contig731sequence in salivary gland transcriptome library of H.flava,the full-length cDNA of cathepsin L-like cysteineproteinaseB(cathepsin L-lcpB)was amplified by RACE,and the expression levels of Hf-CLlCPb in different tissues of female adult ticks in different feeding states were analyzed by qPCR.Results The total length of Hf-CLlCPb was 1547bp,the ORF frame was 1005bp,and it encoded 355amino a...