Characterization of the transcriptional regulator CsbHLH62 that negatively regulates EGCG3\"Me biosynthesis in Camellia sinensis - 成果

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Characterization of the transcriptional regulator CsbHLH62 that negatively regulates EGCG3"Me biosynthesis in Camellia sinensis

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成果类型：

期刊论文

作者：

Luo, Yong;Yu, Shuang-shuang;Li, Juan;Li, Qin;Wang, Kun-Bo*;...

通讯作者：

Wang, Kun-Bo;Huang, Jian-an;Liu, Zhong-hua

作者机构：

[Huang, Jian-an; Yu, Shuang-shuang; Liu, Zhong-hua; Wang, Kun-Bo; Wang, KB; Huang, JA; Li, Qin; Luo, Yong; Li, Juan] Hunan Agr Univ, Minist Educ, Key Lab Tea Sci, Changsha 410128, Hunan, Peoples R China.

[Huang, Jian-an; Liu, Zhong-hua; Wang, Kun-Bo; Li, Qin; Li, Juan] Hunan Agr Univ, Natl Res Ctr Engn & Technol Utilizat Bot Funct In, Changsha 410128, Hunan, Peoples R China.

[Huang, Jian-an; Liu, Zhong-hua; Wang, Kun-Bo; Li, Qin; Li, Juan] Hunan Agr Univ, Hunan Coinnovat Ctr Utilizat Bot Funct Ingredient, Changsha 410128, Hunan, Peoples R China.

通讯机构：

[Wang, KB; Huang, JA; Liu, ZH] H

Hunan Agr Univ, Minist Educ, Key Lab Tea Sci, Changsha 410128, Hunan, Peoples R China.

语种：

英文

关键词：

CCoAOMT;Camellia sinensis;CsDFR;CsLAR;EGCG3"Me biosynthesis;bHLH

期刊：

Gene

ISSN：

0378-1119

年：

2019

卷：

699

页码：

8-15

DOI：

10.1016/j.gene.2019.03.002

基金类别：

National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31470692, 31670691, 31500567]

机构署名：

本校为第一且通讯机构

院系归属：

园艺园林学院

摘要：

Epigallocatechin-3-O-(3-O-methyl) gallate (EGCG3”Me) in tea (Camellia sinensis (L.) O. Kuntze) is a major source of O-methylated catechin and renowned for a wide range of health effects. However, the transcriptional regulation mechanisms of EGCG3”Me biosynthesis remain unclear. In the present work, the basic Helix-Loop-Helix (bHLH) transcription factor, designated as CsbHLH62, belonging to GBOF group of bHLH families, was isolated and characterized from Camellia sinensis. CsbHLH62 contains an Open Reading Frame of 1662 bp and encodes a polype...

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Characterization of the transcriptional regulator CsbHLH62 that negatively regulates EGCG3"Me biosynthesis in Camellia sinensis

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