期刊论文

中文

南昌航空大学学报(自然科学版)

2096-8566

2023

37

02

63-69

10.3969/j.issn.2096-8566.2023.02.008

42007431:国家自然科学基金 19k045:湖南省高校创新平台开放基金项目 EA202002153:南昌航空大学博士启动基金

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通过平板分离法和刚果红染色法,从腐烂苹果中分离得到1株碱性果胶酶产生菌株Lys-3004。经形态观察和16S rDNA扩增子测序分析,确定该菌株为芽孢杆菌属(Bacillus sp.Lys-3004)。通过单因素及正交试...展开更多 通过平板分离法和刚果红染色法,从腐烂苹果中分离得到1株碱性果胶酶产生菌株Lys-3004。经形态观察和16S rDNA扩增子测序分析,确定该菌株为芽孢杆菌属(Bacillus sp.Lys-3004)。通过单因素及正交试验优化产酶条件,结果表明:菌株Lys-3004在7.0%葡萄糖、1.2%黄豆粕、培养基初始pH 7.0、33℃、180 r/min恒温震荡培养96 h的条件下,发酵产生的果胶酶酶活力达到11352.5 U/mL,较优化之前提高了3.7倍...

An alkaline pectinase producing strain Lys-3004 was isolated from a decayed apple by combination of plate screening and Congo red staining.Based on morphological characteristics and 16S rDNA amplicon sequencing analysis,strain Lys-3004 was identified as Bacillus sp.The ...MORE An alkaline pectinase producing strain Lys-3004 was isolated from a decayed apple by combination of plate screening and Congo red staining.Based on morphological characteristics and 16S rDNA amplicon sequencing analysis,strain Lys-3004 was identified as Bacillus sp.The op...