期刊论文

中文

高技术通讯

1002-0470

2001

11

4

1-5

10.3321/j.issn:1002-0470.2001.04.001

(863-101-05-05-04):国家高技术研究发展计划(863计划)

本校为第一机构

通过分子重组,将人#alpha#-干扰素(hu-IFN-#alpha#)基因编码序列克隆到鲤鱼#beta#-肌动蛋白基因启动子下游,构建成能在鱼体内组成型表达hu-IFN-#alpha#的基因重组分子。经限制性酶切分析、Southern杂交和序列测定,证明重组分子构建的正确性。采用显微注射法将这一重组基因转化草鱼受精卵,获得了表达人#alpha#-干扰素的转基因草鱼。

The coding sequence of hu-IFN-α gene is cloned under the control of Carp β-actin gene promoter. Thus a fish constantly expression of hu-IFN-α gene recombinant is constructed. Southern blot and sequencing results show that the recombinant molecule is correctly constructed. The recombinant gene is transferred into fertilized zygotes of grass carp via microinjection. And hu-IFN-α i...