期刊论文

中文

生态环境学报

1674-5906

2010

19

3

576-579

10.16258/j.cnki.1674-5906.2010.03.013

湖南农业大学青年基金项目（07QN06） 湖南农业大学动物医学院青年基金项目（BK0718）

本校为第一机构

以仔猪睾丸支持细胞为实验模型,采用二步酶消化法分离支持细胞进行培养.探讨了0、10、20、40、80 μmol·L~(-1)的氯化镉对支持细胞的毒性作用.结果表明:10 μmol·L~(-1).以上的氯化镉有抑制支持细胞生长的作用,并能使支持细胞氧化酶活性下降,造成支持细胞DNA损伤.

The study was designated to explore the toxic effects of cadmium on piglet Sertoli cells. Sertoli cells were isolated from piglet testes uisng a two-step enzyme digestion and followed by differential plating. Piglet Sertoli cells were cultured and classified into five groups, i.e, group A, control without cadmium chloride; group B with 10μM cadmium chloride; group C with 20μM cadmium chloride; group D with 40μM cadmium chloride; and group E with 80 μM cadmium chloride. We found that Sertoli cells＇ growth was inhibited by arsanilic acid at ...