期刊论文

Junhua Chen

英文

Talanta

0039-9140

2023

255

124210

10.1016/j.talanta.2022.124210

Credit author statement Jiafeng Pan: Conceptualization, Methodology, Data curation, Writing-original draft. Fang Deng: Conceptualization, Methodology, Validation, Writing-original draft. Zhi Liu: Methodology, Data curation, Validation. Lingwen Zeng: Methodology, Formal analysis, acquisition. Junhua Chen: Conceptualization, acquisition, Project administration, Supervision, Writing-review & editing.

本校为第一机构

We successfully constructed several molecular logic gates using heavy metal ions as inputs based on catalytic hairpin assembly (CHA) and CRISPR-Cas12a. The corresponding DNAzymes were used to recognize heavy metal ions (Hg(2+), Cd(2+), Pb(2+), and Mn(2+)). The specific cleavage between heavy metal ions and DNAzymes leads to the release of the trigger DNA, which can be used to activate CHA through logic computation. The CHA-generated DNA duplexes contain the protospacer adjacent motifs (PAM) sequence, which can be distinguished by CRISPR-Cas12a. The hybridization interactions between the duplex...