A total of 3 bacterial strains were obtained from the sick Rana catesbeiana liver, limb, ascites, and other tis-sues. Consistency of their physical and chemical properties was confirmed by the observation of colony morphology, Gram staining, physiological and biochemical identification, and they were all Acinetobacter lwoffi. Two different artificial infec-tion ways of abdominal injection and soak confirmed that the bacteria remained pathogenic. Drug sensitive tests showed that the strain W2011-01 was sensitive to 18 kinds of antibiotics, which were Levofloxacin, Meropenem, Piperacillin/Tazoba...