期刊论文

Duan, Meijuan;Yuan, Dingyang

英文

FRONTIERS IN PLANT SCIENCE

1664-462X

2020

11

537535

10.3389/fpls.2020.00619

We thank Prof. Yaoguang Liu (South China Agricultural University) provided the CRISPR/Cas9 editing vectors, pYLgRNA-U3, and pYLCRISPR/Cas9-MH, Prof. Kaijun Zhao (Chinese Academy of Agriculture Sciences) and Dr. Yan Peng (Hunan Agricultural University) for their suggestions for improvement of the manuscript. Funding. This work was supported by Entrustment Project of Hunan Province (Grant No. 2019TJ-Q08), by National Transgenic Projects of China (Grant No. 2014ZX0801001B and No. 2016ZX08001-001), by the National Natural Science Foundation of China (Grant No. 31771767 and No. 31801341), by Hunan Science and Technology Major Project (Grant No. 2018NK1010).

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“Easy-to-shatter” trait is a major cause of rice crop yield losses, emphasizing the economic value of developing elite rice cultivars with reduced seed shattering capable of achieving higher yields. In the present study, we describe the development of new indica rice lines that exhibit lower rates of seed shattering following the targeted CRISPR/Cas9-mediated editing of the qSH1 gene. We were able to identify qSH1 mutant T0 transgenic plants, with transgene-free homozygous mutants being obtained via segregation in the T1 generation. We then u...