期刊论文

Determination of the Monosaccharide Components, Physicochemical Property and Antioxidant Activity of Polysaccharides from Orange Peel

中文

广州化工

1001-9677

2016

1

125-128

10.3969/j.issn.1001-9677.2016.01.048

2014WK3011:湖南省科技计划 10C0816:教育厅项目 109WD16:湖南农业大学科学基金 DFCXY201219:大学生科技创新项目 :湖南中烟工业有限责任公司合作项目

本校为第一机构

探索并优化了橙皮多糖的提取工艺条件,检测纯化后橙皮多糖的组成,理化性质及抗氧化性；方法：采用用单因素及正交实验设计探索并优化超声波辅助提取橙皮多糖的工艺条件,粗多糖经氯仿：正丁醇(5：1, V：V)去蛋白、浓缩、乙醇沉淀后冷冻干燥,利用离子色谱技术分析纯化后橙皮多糖的组成,采用常规理化检测方法及体外抗氧化实验检测了纯化后橙皮多糖的理化性质及抗氧化性能力。结果表明离子色谱法测得橙皮多糖由3种单糖组成,分别为鼠李糖、葡萄糖、半乳糖醛酸；理化性质实验结果显示,橙皮多糖溶于水,微溶于体积分数为95%乙醇,不溶于乙醚、丙酮,灰分含量为0.1191%,蛋白质含量为0.15 mg/g抗氧化能力低于...

Extracting condition, composition, physicochemical property and antioxidant activity of polysaccharide which was extracted and purified from orange peel were investigated. Polysaccharide from orange peel was extracted using ultrasonic, and optimum extracting conditions of polysaccharide from orange peel with ultrasonic were studied by one- factor-at-a-time method and orthogonal experiments. The crude polysaccharide was purified by eliminating proteins with chloroform： n-butanol （5 ： 1, V ： V） , concentrating, precipitating with cold ethano...