期刊论文

中文

湖南农业科学

1006-060X

2008

2

21-23

10.3969/j.issn.1006-060X.2008.02.008

湖南省教育厅项目（63020152）

本校为第一机构

以带腋芽的茎段为外植体,研究了南方红豆杉组培增殖和植株再生的条件。结果表明：3月份采集的处于旺盛生长期的嫩枝最适宜腋芽的增殖培养;腋芽萌发及生长的最佳培养基为DCR＋0.1 mg/LIBA＋0.05 mg/L BA;最佳的生根培养基为White＋0.2 mg/LIBA。当根长约0.5 cm时,移栽至营养钵中,在温度25℃,75%以上湿度下栽培30 d左右,逐渐加大光照强度,植株成活率可达90.1%。

The regeneration system of Taxus chinensis Var. Mairei was studied using stems with axillary buds as explants. The results showed that the optimal explants were young twigs in the growing state. The optimal medium of axillary buds germination and growth was DCR ＋ IBA 0.1 mg/L ＋ BA 0.05 mg/L. The optimal medium of roots was White ＋ IBA 0.2 mg/L. Trans - plant seedlings into pan with nutrition when the length of root was 0.5 cm, temperature 25 ℃, humidity above 75 %, gradually increased the intensity of illuminati...