期刊论文

中文

湖南农业科学

1006-060X

2009

4

5-7,10

10.3969/j.issn.1006-060X.2009.04.002

2006AA02Z155:国家高技术研究发展计划（863计划）

本校为第一机构

采用正交试验对1株基因工程菌株的种子培养基与发酵培养基进行优化.通过在3因素(碳源、生长素、无机盐)3水平上进行正交试验,确定种子培养基成分为:0.5%牛肉膏,0.5%葡萄糖,0.5%蛋白胨,0.35%NaCl,0.1%KH_2PO_4,0.05%MgSO_4;发酵培养基的成分为:0.5%牛肉膏,0.3%魔芋精粉,0.5%蛋白胨,0.35%NaCl,0.1%KH_2PO_4,0.05%MgSO_4;产酶高峰时段为10～12 h之间,最适的发酵温度为35℃,最高酶活可达775 U/ml.

Uses the orthogonal test the method the seed culture medium which and the fermentation medium ferments to a genetic engineering strain carries on the optimization. Through in three factors （carbon source, growth hormone, inorganic salt） in three levels carries on the orthogonal test, the examination seed culture medium fungus hangs the fluid density （spectrophotometric method to measure that OD660） compares its growth situation, thus the determination seed culture medium ingredient is： 0.5% cow meat extract, 0.5% glucose, 0.5% protein pept...