期刊论文

中文

湖南农业科学

1006-060X

2010

15

128-130

10.3969/j.issn.1006-060X.2010.15.042

2008BAD92B06:国家科技支撑计划 2007A020200003-3:广东省科技攻关计划 2009DFA31470:国家国际科技合作专项基金 948;2008-G1:国家项目

本校为第一机构

以香蕉栽培种"过山香"(Musa AAB Silk cv. Guoshanxiang)和野生阿宽蕉(Musa itinerans Cheesm. AB Group)原生质体为融合试材,进行香蕉高效融合的电融合参数优化.研究表明,在交变电场(AC)200 V/cm,AC作用时间30 s,直流脉冲电压(DC)1500 V/cm,DC脉冲时间40μs,脉冲次数为2次时,融合效率最佳,融合率可达32.4%以上,原生质体活力达81.4%

In order to optimize the parameters of banana＇s electrofusion,the protoplasts that isolated from Musa AAB Silk cv.Guoshanxiang and Musa itinerans Cheesm.AB Group were taken as material of electrofusion.It was shown that when electrofusion parameters were alternate current（AC） 200 V/cm,AC duration 30 s,direct current（DC） 1 500 V/cm,DC pulse with 40 μs and 2 times,the fusion efficiency was the best,which electrofusion frequency reache...