A comparative study was conducted to the esterase isozymes in Pardosa pseudoannulata at different growth periods under application of trichlorfon pesticide by using the vertical slab polyacrylamide gel electrophoresis technology.The results showed that there were significant differences between esterase isozymes in different growth periods;the number of isozyme band showed a trend of less-more-less,and it reached peak at 6th instar;the concentration of isozymes increased with the increasing number of isozyme band in Pardo...