期刊论文

Separation,Purification and Enzymatic Properties of Butanol Dehydrogenase in Clostridium acetobutylicum

中文

湖南农业科学

1006-060X

2011

07

18-20,25

10.3969/j.issn.1006-060X.2011.07.006

本校为第一机构

对丙酮丁醇梭状芽孢杆菌（Clostridium acetobutylicum）中丁醇代谢途径相关的关键酶之一依赖NADH的丁醇脱氢酶（Butanol Dehydrogenase,BDH）的分离纯化及酶学性质进行了研究。结果表明:粗酶液经过两次饱和度为20%和60%的硫酸铵沉淀和SephadexG-75凝胶柱层析,丁醇脱氢酶的纯化倍数提高至12.7倍,回收率为28.9%。丁醇脱氢酶的最适反应温度是35℃,最适pH是6.0。

The separation,purification and enzymatic properties of NADH dependent Butanol Dehydrogenase（BDH）,which is one of the key enzymes relates to the metabolic pathway of butanol metabolismin in Clostridium acetobutylicum,were studied.The results showed that after the crude enzyme solution was purified by ammonium sulfate precipitation with 20% saturation degree and 60% saturation degree and SephadexG-75 gel column chromatography,the purification fold of butanol dehydrogenase reached 12.7-fold,and recovery rate was 28.9%.The optimal temperature and opti...