期刊论文

Optimization of a Bacillus Strain X23 Fermentation Medium and Property of Its Antibacterial Substances

中文

湖南农业科学

1006-060X

2014

11

1-2+12

10.3969/j.issn.1006-060X.2014.11.001

本校为第一机构

通过平板对峙培养试验发现短短芽孢杆菌X23对细菌、真菌、卵菌等多种植物病原菌都有拮抗作用,在NB培养基基础上通过单因素变量法进行优化,并对发酵液稳定性进行研究。结果表明:当培养基中牛肉膏1 g/L,蛋白胨5 g/L,葡萄糖10 g/L时,发酵液抑菌效果最好。60和70℃加热处理1 h的发酵液抑菌活性不变,80℃以上抑菌活性下降,100℃以上活性丧失;不同pH值中过夜处理,抑菌活性不降低;紫外光照28h抑菌活性不变,10 h以上活性下降。试验证明抑菌活性物质具有良好的耐热性,耐酸碱性以及紫外稳定性。

The fermentation broth of strain X23 has found possess a strong inhibiting effect for many plant pathogens, through confrontating incubation on plates. For further study, the NB medium has been improved with single factor analysis method. Through the comparison of bacteriostatic ring, the best inhibiting effect was obtained when beef extract is of 1 g/L, peptone of 5g/L and glucose of 10g/ L. Heat treatment in 60℃ and 70℃ for 1 h, the bacteriostatic activity of X23 is changeless, but will decline more than 80 ℃ and loss above 100℃; the chan...