In this study, a type of 10 m2 layered circuit pedrail fermentation box was designed and manufactured, and its temperature, humidity, aseptic performance and fermentation effect were measured. The fermentation box is composed of three parts such as box body, fermentation bed and auxiliary facilities. The box body is made of fireproof color steel. The fermentation bed is made of stainless steel with a double-bed and three-layer structure. Auxiliary equipment is mainly composed of purified air supply and heating system, fan system, spray conditioning system, and disinfection and sterilization sy...