期刊论文

中文

湖南农业科学

1006-060X

2024

05

1-6

10.16498/j.cnki.hnnykx.2024.005.001

2016NK2114:湖南省农业领域重点研发项目

本校为第一机构

以硝化纤维素为筛选培养基,对采样分离所得菌株和实验室保藏菌株进行平板筛选,获得长势优良的 6 株降解菌;经固态发酵复筛,获得硝化纤维素降解菌TL06;经ITS序列分析及构建进化树分析,TL06 被鉴定为西蒙斯木霉,且命名为西蒙斯木霉TL06.分别探究培养基成分、培养条件对西蒙斯木霉TL06 产生纤维素酶和酯酶活力的影响,结果表明:培养基中添加有机氮源会显著降低纤维素酶和酯酶活力,而添加无机氮源硫酸铵会显著提高纤维素酶活力;添加碳源会显著降低纤维素酶活力,但对酯酶活力影响不明显.为优化发酵条件设置正交试验,发现在硫酸铵浓度 1.5%、硝化纤维素与麸皮的质量比 5∶5、发酵时间 5 d、料水比 10∶...

Nitrocellulose was used as a screening medium,and six degrading strains with excellent growth were obtained by plate screening of the isolated strains after sampling and the strains stored in the laboratory.The nitrocellulose degrading strain TL06 was obtained by solid state fermentation and secondary screening.Through ITS sequence analysis and phylogenetic tree analysis,it was identified as Trichoderma simmonsii and named Trichoderma simmonsii TL06.The effects of medium components and culture conditions on cellulase and esterase activities of ...