A cascade toehold-mediated strand displacement strategy for label-free and sensitive non-enzymatic recycling amplification detection of the HIV-1 gene

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成果类型：

期刊论文

作者：

Li, Qiong;Liu, Zhi;Zhou, Danhua;Pan, Jiafeng;Liu, Chengshuai;...

通讯作者：

Chen, Junhua

作者机构：

[Liu, Zhi; Li, Qiong] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.

[Zhou, Danhua; Pan, Jiafeng; Chen, Junhua; Li, Qiong] Guangdong Inst Ecoenvironm & Sci & Technol, Guangdong Key Lab Integrated Agroenvironm Pollut, Guangzhou 510650, Guangdong, Peoples R China.

[Zhou, Danhua] South China Agr Univ, Coll Nat Resources & Environm, Guangzhou 510462, Guangdong, Peoples R China.

[Liu, Chengshuai] Chinese Acad Sci, Inst Geochem, State Key Lab Environm Geochem, Guiyang 550081, Guizhou, Peoples R China.

通讯机构：

[Chen, Junhua] G

Guangdong Inst Ecoenvironm & Sci & Technol, Guangdong Key Lab Integrated Agroenvironm Pollut, Guangzhou 510650, Guangdong, Peoples R China.

语种：

英文

期刊：

ANALYST

ISSN：

0003-2654

年：

2019

卷：

144

期：

页码：

2173-2178

DOI：

10.1039/c8an02340a

基金类别：

Guangdong National Science Funds for Distinguished Young Scholars [2016A030306012]; NSFCNational Natural Science Foundation of China (NSFC) [31671933]; Local Innovative and Research Teams Project of the Guangdong Pearl River Talents Program [2017BT01Z176]

机构署名：

本校为第一机构

院系归属：

生物科学技术学院

摘要：

In this work, a label-free fluorescence biosensor for simple detection of the HIV-1 gene was proposed by using toehold-mediated strand displacement reactions (TMSDRs) combined with a non-enzymatic target recycling amplification strategy. In this system, two TMSDRs were used. In the presence of the HIV-1 gene, an autocatalytic DNA machine can be activated. This leads to the generation of numerous free G-rich sequences, which can associate with a fluorescent dye N-methylmesoporphyrin IX (NMM) to yield an amplified fluorescence signal for the target detection. This sensing platform showed a high ...

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A cascade toehold-mediated strand displacement strategy for label-free and sensitive non-enzymatic recycling amplification detection of the HIV-1 gene

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