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A binding protein regulates myosin-7a dimerization and actin bundle assembly

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成果类型:
期刊论文
作者:
Liu, Rong;Billington, Neil;Yang, Yi;Bond, Charles;Hong, Amy;...
通讯作者:
Sellers, James R
作者机构:
[Hong, Amy; Liu, Rong; Siththanandan, Verl; Billington, Neil; Bond, Charles; Yang, Yi; Takagi, Yasuharu; Sellers, James R.] NHLBI, Lab Mol Physiol, NIH, Bldg 10, Bethesda, MD 20892 USA.
[Yang, Yi] Hunan Agr Univ, Coll Vet Med, Lab Funct Prote, Changsha 410128, Hunan, Peoples R China.
通讯机构:
[Sellers, James R] L
Laboratory of Molecular Physiology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD, 20892, USA.
语种:
英文
期刊:
Nature Communications
ISSN:
2041-1723
年:
2021
卷:
12
期:
1
页码:
563
基金类别:
We thank the Electron Microscopy, Light Microscopy, Proteomics and Biophysics Core Facilities of the National Heart, Lung, and Blood institute (NHLBI) for their support, advice, and the use of facilities. We thank the laboratory of Philipp Kukura for the mass photometry technology. We thank Fang Zhang for her help with reagent and actin preparation. R.L. is supported by a postdoctoral fellowship from NHLBI (Lenfant Biomedical Fellowship). This work is also supported by the Intramural Research Program of the NHLBI, NIH (HL006049 to J.R.S.).
机构署名:
本校为其他机构
院系归属:
动物医学院
摘要:
Myosin-7a, despite being monomeric in isolation, plays roles in organizing actin-based cell protrusions such as filopodia, microvilli and stereocilia, as well as transporting cargoes within them. Here, we identify a binding protein for Drosophila myosin-7a termed M7BP, and describe how M7BP assembles myosin-7a into a motile complex that enables cargo translocation and actin cytoskeletal remodeling. M7BP binds to the autoinhibitory tail of myosin-7a, extending the molecule and activating its ATPase activity. Single-molecule reconstitution show that M7BP enables robust motility by complexing wit...

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