期刊论文

Bioinformatics Analysis on Gene Coding for the Low Molecular Weight Glutenin in HeDong WuMai

中文

山东化工

1008-021X

2019

48

2

28-32

10.19319/j.cnki.issn.1008-021x.2019.02.011

13JJ3116:湖南省自然科学基金 14YB08:湖南文理学院科学研究项目

本校为第一机构

低分子量谷蛋白是一种重要的贮藏蛋白质。它对小麦的加工品质有重大影响,尤其在面点制作过程中具有较大作用。本研究通过提取纯化河东乌麦总基因组DNA,利用特异引物进行扩增,扩增产物经回收纯化后送生物公司测序。生物信息学软件分析发现,得到1条长度为918bp的低分子量谷蛋白编码基因序列,其上共有18个酶切位点,GC含量为49.0%。该基因与来自于普通小麦低分子量麦谷蛋白得亚基GluA3-21和GluA3-1基因相似值达到99%。该基因编码306个氨基酸,分子量大小为34588.0kDa,信号肽序列为MKTFLVFALLAVVATSAIA。Gln谷氨酰胺含量最高,为35.00%,二级结构主要为无规则卷曲结构组成,其次为片层结构。本研究结果可...

The low molecular weight glutenin (LMW-GS) is a kind of important storage proteins.It has significant influence on the final processing quality of wheat, especially in Asia pastries production process are useful.We studied a good cultivar Hedong Wu Mai, which was bred by Shanxi academy of agricultural science.Wheat DNA was extracted and purified, use specific primers for amplification, after a series of steps, the product was sequenced by biological company and further analyzed by bioinformatics software.A 918 bp low molecular weight protein co...