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Isolation of a feather-degrading strain of bacterium from spider gut and the purification and identification of its three key enzymes

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成果类型:
期刊论文
作者:
Qu, Fang;Chen, Qingwang;Ding, Yiying;Liu, Zihao;Zhao, Yan;...
通讯作者:
Chen, Jinjun
作者机构:
[Liu, Zihao; Chen, Qingwang; Chen, Jinjun; Ding, Yiying; Zhang, Xuewen; Qu, Fang; Zhao, Yan; Liu, Zikui] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.
通讯机构:
[Chen, Jinjun] H
Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.
语种:
英文
关键词:
Stenotrophomonas maltophilia;Feather degradation;Spider gut;Synergic enzymes;Symbiotic microorganism
期刊:
Molecular Biology Reports
ISSN:
0301-4851
年:
2018
卷:
45
期:
6
页码:
1681-1689
基金类别:
Educational Department Foundation of Hunan Province [16A098]; Key R & D project of Hunan Provincial Science and Technology Department [2017NK2311]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31772865]
机构署名:
本校为第一且通讯机构
院系归属:
生物科学技术学院
摘要:
A novel feather-degrading bacterium named CA-1 was isolated from the gut of the spider Chilobrachys guangxiensis, which degrades native whole chicken feathers within 20 h. The CA-1 was confirmed to belong to Stenotrophomonas maltophilia based on morphologic and molecular analysis. Maximum feather degradation activity of the bacterium was observed at 37 A degrees C in basal feather medium (NaCl 0.5 g/L, KH2PO4 0.3 g/L, K2HPO4 0.4 g/L, feather powder 10.0 g/L, pH 8.0), which was inhibited when glucose and ammonium nitrate were added in the medium. Furthermore, the purified enzymes under the opti...

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