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Isolation of a feather-degrading strain of bacterium from spider gut and the purification and identification of its three key enzymes

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成果类型:
期刊论文
作者:
Qu, Fang;Chen, Qingwang;Ding, Yiying;Liu, Zihao;Zhao, Yan;...
通讯作者:
Chen, Jinjun
作者机构:
[Liu, Zihao; Chen, Qingwang; Chen, Jinjun; Ding, Yiying; Zhang, Xuewen; Qu, Fang; Zhao, Yan; Liu, Zikui] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.
通讯机构:
[Chen, Jinjun] H
Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.
语种:
英文
关键词:
Stenotrophomonas maltophilia;Feather degradation;Spider gut;Synergic enzymes;Symbiotic microorganism
期刊:
Molecular Biology Reports
ISSN:
0301-4851
年:
2018
卷:
45
期:
6
页码:
1681-1689
基金类别:
Acknowledgements This work was supported by grants from the Educational Department Foundation of Hunan Province (Grant No. 16A098), Key R & D project of Hunan Provincial Science and Technology Department (Grant No. 2017NK2311) and National Natural Science Foundation of China (Grant No. 31772865). We thank Dr. Fangjia Lu in Purdue University for the professional edit. This work was supported by grants from the Educational Department Foundation of Hunan Province (Grant No. 16A098), Key R & D project of Hunan Provincial Science and Technology Department (Grant No. 2017NK2311) and National Natural Science Foundation of China (Grant No. 31772865). We thank Dr. Fangjia Lu in Purdue University for the professional edit. The authors have no conflict of interest to declare.
机构署名:
本校为第一且通讯机构
院系归属:
生物科学技术学院
摘要:
A novel feather-degrading bacterium named CA-1 was isolated from the gut of the spider Chilobrachys guangxiensis, which degrades native whole chicken feathers within 20 h. The CA-1 was confirmed to belong to Stenotrophomonas maltophilia based on morphologic and molecular analysis. Maximum feather degradation activity of the bacterium was observed at 37 A degrees C in basal feather medium (NaCl 0.5 g/L, KH2PO4 0.3 g/L, K2HPO4 0.4 g/L, feather powder 10.0 g/L, pH 8.0), which was inhibited when glucose and ammonium nitrate were added in the medium. Furthermore, the purified enzymes under the opti...

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