期刊论文

中文

南方农业学报

2095-1191

2015

46

11

1943-1948

10.3969/j:issn.2095-1191.2015.11.1943

31271789:国家自然科学基金 20124320110014:高等学校博士学科点专项科研基金 1016DB0316:柳州市横向项目

本校为第一机构

【目的】利用ISSR标记分析柳州九万山野生茶树种质资源遗传多样性和亲缘关系,为柳州九万山野生茶树种质资源的保护和开发利用提供参考依据。【方法】选取10条扩增条带清晰、多态性高、重复性好的ISSR引物,对93株柳州九万山野生茶树单株及6个对照茶树品种(系)的遗传多样性和亲缘关系进行分析。【结果】10条引物共扩增获得112条谱带,其中105条具有多态性,占93.75%;每条引物扩增条带数为7~16条,平均多态性带10.5条;每条引物扩增的多态性带比率为80.00%~100.00%,扩增条带大小在250~2000 bp。供试材料的相似系数介于0.23~0.82,平均为0.51。采用UPGMA法进行聚类分析,结果表明,在相似系数0.51处,供试单...

[Objective]The genetic diversity and relationship of wild tea germplasm resources from Liuzhou Jiuwan Mountain were studied by using ISSR markers in order to provide references for their development,utilization and protection.[Method]The 10 ISSR primers with clear,highly polymorphic,reproducible amplified bands were selected to analyze the genetic diversity and relationship amongst 93 accessions of Liuzhou Jiuwan Mountain wild tea individuals and 6 control tea varieties（lines）.[Result]A total of 112 bands were amplified,of which 105 bands wer...