期刊论文

Effects of SPS activity variation on carbon and nitrogen metabolism,and oil formation in rape silique

中文

南方农业学报

2095-1191

2018

49

2

234-238

10.3969/j.issn.2095-1191.2018.02.05

SQ2017YFNC050022:国家重点研发计划 SX2017B390:湖南省研究生科研创新项目

本校为第一机构

【目的】探明油菜角果蔗糖磷酸合成酶(Sucrose phosphate synthase,SPS)活性变化对其碳氮代谢产物及油分含量的影响,为进一步研究油菜SPS表达调控机理、改良油菜品质和产量的矛盾提供理论依据。【方法】以氮低效和氮高效油菜品种为试验材料,在正常供氮和氮胁迫条件下进行土培试验。角果发育中期涂抹SPS抑制剂,以涂抹蒸馏水为对照,处理后第3 d上午测定角果皮及籽粒的SPS活性、可溶性糖和游离氨基酸含量,成熟期测定籽粒油分含量。【结果】角果涂抹SPS抑制剂后,不同氮水平条件下不同氮效率油菜品种的SPS活性均明显下降,其差异达显著水平(P

[Objective]Effects of sucrose phosphate synthase(SPS)activity variation in rape silique on carbon and ni-trogen metabolite and rapeseed oil content were studied to provide reference for revealing the expression regulation mecha-nism of SPS,and contradiction between quality and yield of improved rape varieties.[Method]Rape varieties with low ni-trogen use efficiency and high nitrogen use efficiency were used in this experiment,and soil culture test under normal ni-trogen supply and nitrogen stress was carried out.During silique development period,rape siliques were smeared with SPS inhibitor,an...