期刊论文

Effects of Bacillus subtilis M-23 on Diaporthe citri and diversity of bacterial community in the citrus phyllosphere

中文

南方农业学报

2095-1191

2020

51

7

1699-1705

10.3969/j.issn.2095-1191.2020.07.024

现代农业（柑橘）产业技术体系岗位科学家项目（CARS-26）； 湖南省水果产业技术体系柑橘植保岗位专家项目（湘农发〔2015〕137号）； 湖南农业大学双一流建设子项目（SYL2019027）;湖南农业大学青年科学基金项目（19QN30）~~；

本校为第一机构

[目的]明确柑橘砂皮病不同发病等级及喷施生防菌枯草芽孢杆菌M-23后柑橘叶际细菌群落结构的变化,揭示生防菌防治柑橘砂皮病的叶际微生物学机制,为进一步研究柑橘砂皮病的生物防治提供理论基础.[方法]试验设对照区和喷施生防菌区2个区域,喷施生防菌区分别在2019年3月中旬柑橘春梢长2～3 cm、4月中旬、谢花2/3、果实蚕豆大小和果实乒乓球大小时喷施M-23菌株发酵液,喷药液量3 L/株;同时对照区喷施等量的灭菌NB液体培养基,在最后一次喷施生防菌15 d后进行防效调查.采集喷施生防菌区柑橘叶片(R4处理,病情等级0级)和未喷施生防菌区不同发病等级的柑橘叶片(R1、R2和R3处理,病情等级分别为0、5和9级),使...

【Objective】Focusing on different severities of Diaporthe citri and the shifts of bacterial community diversity in citrus phyllosphere after spraying with Bacillus subtilis M-23,this study was to reveal the phyllospheric microbiological mechanism of controlling D.citri with biocontrol bacteria and provide theoretical basis for further studies on biological control of D.citri.【Method】The experiment was conducted in two areas—the control area and the treatment area.In the treatment area,biocontrol bacteria M-23 was sprayed in the middle of Ma...