Development of transgenic CryIA(c) + GNA cotton plants via pollen-tube pathway method confers resistance to Helicoverpa armigera and Aphis gossypii glover

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成果类型：

期刊论文

作者：

Liu, Z.;Zhu, Z.;Zhang, T.*

通讯作者：

Zhang, T.

作者机构：

College of Bioscience and Biotechnology, Hunan Agricultural University, Hunan, China

Genetics and Developmental Biology Institute, Chinese Academy of Sciences, Beijing, China

Agronomy Department, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, Zhejiang, China

National Key Laboratory of Crop Genetics and Germplasm Enhancement, Cotton Research Institute, Nanjing Agricultural University, Nanjing, China

通讯机构：

Agronomy Department, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, Zhejiang, China

语种：

英文

关键词：

Aphis gossypii Glover;CryIA(c);GNA;Helicoverpa armigera;Insect resistance;Pollen-tube pathway

期刊：

Methods in Molecular Biology

ISSN：

1064-3745

年：

2019

卷：

1902

页码：

233-244

DOI：

10.1007/978-1-4939-8952-2_20

机构署名：

本校为第一机构

院系归属：

生物科学技术学院

摘要：

Elite cotton cultivar Sumian16 was transformed with p7RPSBK-mGNA-NPTII containing Bt (CryIA(c)), Galanthus nivalis agglutinin (GNA) resistance genes, and selectable marker NptII gene via the pollen-tube pathway method, and two fertile transgenic Bt + GNA plants were obtained in the present study. The integration and expression of the Bt and GNA genes were confirmed by molecular biology techniques and insect bioassays. Insect bioassays showed that the transformed plants were highly toxic to bollworm larvae as well as obviously retarding developm...

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Development of transgenic CryIA(c) + GNA cotton plants via pollen-tube pathway method confers resistance to Helicoverpa armigera and Aphis gossypii glover

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