The lack of sophisticated in vitro models limits our current understanding of gastrointestinal functions in farm animals. Conventional 2D cell lines or primary cells fail to recapitulate the physiology of in vivo intestinal epithelium. In contrast stem cell-derived, nontransformed 3D enteroids partially recreate the villus-crypt anatomy of the native intestine and comprise most if not all intestinal cell types including enterocytes, enteroendocrine cells, goblet cells, Paneth cells, and stem cells. This review summarizes the techniques used for generating and culturing enteroids of various far...
