Identification of WA-type three-line hybrid rice with real-time Polymerase Chain Reaction (PCR) method

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成果类型：

期刊论文、会议论文

作者：

Cheng, Y.*;Gao, B. D.;Chen, H. Y.;Mao, J. J.;Cao, A. X.;...

通讯作者：

Cheng, Y.

作者机构：

[Cheng, Y.] Chinese Acad Agr Sci, Inst Bast Fiber Crops, Changsha, Hunan, Peoples R China.

[Chen, H. Y.; Cao, A. X.; Mao, J. J.; Zhu, S. F.] Chinese Acad Inspect & Quarantine, Inst Anim & Plant Quarantine, Beijing, Peoples R China.

[Gao, B. D.; Mao, J. J.; Cheng, Y.] Hunan Agr Univ, Coll Biosafety Sci & Technol, Changsha, Hunan, Peoples R China.

[Zhu, J. G.] Hunan Entry Exit Inspect & Quarantine Bur, Changsha, Hunan, Peoples R China.

[Cheng, Y.] Chinese Acad Agr Sci, Inst Bast Fiber Crops, Xianjiahu W Rd 348, Changsha, Hunan, Peoples R China.

通讯机构：

[Cheng, Y.] C

Chinese Acad Agr Sci, Inst Bast Fiber Crops, Xianjiahu W Rd 348, Changsha, Hunan, Peoples R China.

语种：

英文

关键词：

Hybrid rice;Male sterility;Molecular identification;mtDNA;PCR;R2-630 WA;Real-time fluorescent

期刊：

Applied Biochemistry and Biotechnology

ISSN：

0273-2289

年：

2012

卷：

166

期：

页码：

819-829

会议名称：

生命科学前沿与农业生物技术创新全国博士生学术论坛

会议论文集名称：

生命科学前沿与农业生物技术创新全国博士生学术论坛论文集

会议时间：

2012-7-18

会议地点：

武汉

会议主办单位：

华中科技大学

DOI：

10.1007/s12010-011-9471-0

基金类别：

Acknowledgments This work was partly funded by molecular marker system of bio-resources General Administration of Quality Supervision, Inspection and Quarantine of the People’s Republic of China. The authors would like to thank Dr. H.L. Yang from the China National Hybrid Rice Research and Development Center, Dr. W.X. Zhang from the Institute of Crop Germplasm Resource Chinese Academy of Agricultural Sciences (CAAS) and Dr. S.W. Huang from the China National Rice Research Institute for the donation of samples. We are grateful to Professor J. Leng and W. Li from the Institute of Bast-Fiber and L. Yu from Hunan Agricultural University for their critical reading and editing of the manuscript.

机构署名：

本校为其他机构

院系归属：

植物保护学院

摘要：

A real-time fluorescent PCR (RTF-PCR) was developed to detect and quantify wild abortive (WA)-type three-line hybrid rice (Oryza sativa L.). The mitochondrial R2-630WA gene was reported to be closely related to male sterility in plants, and developed as a molecular maker to identify the cytoplasmic male sterility system of hybrid rice. First, we got the DNA sequence of R2-630 WA gene in 17 rice species with traditional PCR. Then, a pair of specific primers (P3, P4) and TaqMan fluorescence probe (P3-14) were designed based on the R2-630 DNA sequ...

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Identification of WA-type three-line hybrid rice with real-time Polymerase Chain Reaction (PCR) method

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