期刊论文

Study on Main Chemical Component of Spongy and Palisade Cells from Different Leaf Position of Tobacco

中文

中国农业科学

Scientia Agricultura Sinica

0578-1752

2007

21

10

2289-2295

10.3321/j.issn:0578-1752.2007.10.023

国家烟草专卖局项目(11020041012)； 云南省烟草公司项目(04A02)； 湖南农业大学校青年基金(60162)；

本校为第一机构

【目的】定量了解不同叶位海绵与栅栏细胞主要品质成分的差异。【方法】采用酶解方法分离‘K326’和红花大金元下部(第4叶)、中部(第10叶)、上部(第16叶)适熟叶片海绵与栅栏细胞,然后通过气相色谱分析鉴定非挥发性有机酸,分光光度计法分析质体色素、糖类及烟碱。【结果】样品海绵与栅栏细胞的数量百分比分别为85%,80%以上;多元有机酸在海绵细胞中含量较高,高级脂肪酸、质体色素、糖类均是栅栏细胞中含量高,而烟碱却是海绵细胞含量高于栅栏细胞;无论是海绵细胞,还是栅栏细胞,上部叶叶绿素含量较高,下部叶非挥发性有机酸含量较高,中部叶类胡萝卜素、总糖和还原糖含量较高,非还原糖与叶位无关。烟碱...

[Objective] The experiment was conducted to study the main quality of different cells of different leaf position from different genotype quantificationally. [Method]Spongy cells and palisade cells of cutter different ripe leaf-position （bottom, 4^th, cutter, 10^th, upper, 16^th） of flue-cured tobacco （＇K326＇ and ＇Hongda＇） were divided by pectinase, then no-volatility organic acid was detected by gas chromatography, and plastid pigment or glucide or nicotine was analyzed by spectrophotometer. [Result] The percent of spongy cells and pali...