期刊论文

中文

中国农业科学

0578-1752

2008

41

11

3542-3548

10.3864/j.issn.0578-1752.2008.11.013

国家自然科学基金（30571186）；

本校为第一机构

[目的]分离苎麻尿苷二磷酸葡萄糖脱氢酶(UDPGDH)基因.[方法]通过简并引物RT-PCR结合RACE 技术和半定量RT-PCR.[结果]成功克隆苎麻Boehmeria nivea(Linn.)Gaud.尿苷二磷酸葡萄糖脱氢酶(UDPGDH)基因cDNA序列,序列长1 837 bp,编码-480个氨基酸的蛋白质(GenBank No.EFl78294).半定量RT-PCR分析显示,UDPGDH在苎麻根、皮、茎和叶组织中均有表达,其表达量为茎>皮>叶>根,相对表达量依次为0.7075,0.3051,0.2651,0.1490.[结论]苎麻UDPGDH与其它植物相应序列具有较高同源性,在苎麻茎中有较高的表达.

[Objective] This research attempted to isolate UDP-glucose dehydrogenase gene in ramie （Boehmeria nivea）. [Method] Degenerate primer was designed to amplify a fragment of UDPGDH gene so as to obtain the full length cDNA using RACE. The expression of UDPGDH gene in different tissues of ramie was analyzed by semi-quantitative RT-PCR. [Result] Full length ramie UDPGDH cDNA of 1 837 bp, encoding protein of 480 amino acids in reading frame （GenBank No. EF178294） was cloned. The expression of UDPGDH was found in leaf, stem, root and phloem in ram...