期刊论文

中文

中国农业科学

0578-1752

2010

43

7

1493-1499

10.3864/j.issn.0578-1752.2010.07.022

农业部科技成果转化基金（20067B2B000048）； 湖南省科技厅重点资助项目（2008NK2007）；

本校为第一机构

[目的]评价头孢噻呋混悬剂对兽医临床常见的3种标准菌株的体外抗菌活性及抗生素后效应.[方法]以试管二倍稀释法对3种标准菌株进行最低抑菌浓度(MIC)和最小杀菌浓度(MBC)的测定;采用菌落计数法绘制3种标准菌株的时间-杀菌曲线(KCs);采用平板计数法测定了头孢噻呋混悬剂对3种标准菌株的抗生素后效应(post-antibiotic effect,PAE).[结果]头孢噻呋混悬剂对大肠杆菌,放线杆菌及猪兽疫链球菌的MIC值分别为:0.076,0.304、0.00475 μg·mL~(-1);MBC值分别为:0.61、0.304、0.00475 μg·mL~(-1);头孢噻呋混悬剂对放线杆菌的杀菌速率最快,对链球菌次之;头孢噻呋混悬剂对大肠杆菌PAE较短,呈现非浓度依赖性,...

[Objective] The objective of the study was to evaluate the in vitro antibacterial activity and post-antibiotic effect of ceftiofur suspension on three standard bacteria strains in veterinary clinic. [Method] Minimal inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were tested by double dilution method to three standard bacteria strains. Time-kill curve was drew according to colony count. Post-antibiotic effect (PAE) was determined by plate count. [Result] MIC value of ceftiofur suspension to E. Coli, Actinobacillus an...