期刊论文

中文

中国农业科学

0578-1752

2010

43

10

2000-2007

10.3864/j.issn.0578-1752.2010.10.004

(2006AA10A112;2006-G04):国家高技术研究发展计划(863计划)

本校为第一机构

目的 建立适合于提取油菜叶片全蛋白的三氯乙酸/丙酮沉淀法,为今后油菜蛋白质组学的研究提供参考,明确菌核菌诱导抗、感菌核病油菜后的蛋白差异表达情况,为寻找抗菌核病基因提供线索.方法 以油菜抗菌核病近等基因系98C40(抗原来自湘油15)和感菌核病亲本98C40为材料,在其生长的苗期阶段,接种菌核菌72 h之后,分别提取其叶片全蛋白.运用双向电泳技术、PD-quest软件、质谱技术对其进行差异蛋白质组分析.结果 检测到抗病98C40(抗原来自湘油15)与感病亲本98C40之间有4个差异蛋白点,蛋白点a,线粒体ATP合酶Fip亚基(mitochondrial Fl ATP synthase beta subunit1);蛋白点c,磷酸甘油酸激酶 (phosphoglycer...

[Objective] A trichoroacetic acid/acetone method was established for extracting the full leaf-proteins of rapeseed which provided the reference for the rapeseed proteomics research in the future. The difference in protein expression was identified after the resistance and susceptibility to Sclerotinia sclerotiorum rapeseed were induced by Sclerotinia sclerotiorum. It offered the clues to searching for the resistance to Sclerotinia sclerotiorum genes. [ Method] Near-isogonic lines 98C40 (the antigen derived from Xiangyou 15) is resistant to Sclerotinia sclerotiorum and the parent 98C40 is susce...