期刊论文

中文

中国农业科学

0578-1752

2013

46

3

534-544

10.3864/j.issn.0578-1752.2013.03.010

国家公益性行业（农业）科研专项（201103018）；

本校为第一机构

【目的】建立一种基于环介导等温扩增（Loop-mediated isothermal amplification，LAMP）技术，从植物罹病组织中直接检测象耳豆根结线虫（Meloidogyne enterolobii）的快速检测方法，为象耳豆根结线虫的监测和防治提供技术支持。【方法】根据象耳豆根结线虫与其它根结线虫ITS序列差异设计LAMP特异性引物，通过对LAMP反应条件中的Mg~(2+)、dNTPs、甜菜碱和反应时间进行优化，同时对检测体系的特异性和灵敏度进行验证，建立一种从罹病植物组织中检测象耳豆根结线虫的LAMP快速检测方法。【结果】象耳豆根结线虫LAMP检测体系优化结果表明在Mg~(2+)的浓度为5.0 mmol?L~(-1)、dNTPs的浓度为2.4 mmol?...

[Objective] The objective of this study is to establish a rapid diagnostic method for Meloidogyne enterolobii from the infected plants based on the loop-mediated isothermal amplification （LAMP）, and to provide technical supports for monitoring and prevention ofM. enterolobii. [Method] The LAMP specific primers were designed according to the distinction of ITS （internal transcribed spacer） sequences between M. enterolobii and other Meloidogyne spp.. The reaction conditions of LAMP, including the concentrations of Mg2＋, dNTPs, betain and rea...