期刊论文

SSR Markers Screening for Identification of Citrus Cultivar and Construction of DNA Fingerprinting Library

中文

中国农业科学

0578-1752

2018

51

15

2969-2979

10.3864/j.issn.0578-1752.2018.15.012

31720103915:国家自然科学基金 111721301092361007:农产品质量安全监管专项 :广西柑橘新品种选育及无病毒苗木繁殖研究特聘专家岗位

本校为第一机构

【目的】筛选出一套SSR核心引物,用于构建柑橘品种的DNA指纹图谱库,为柑橘种苗认证、品种登记和植物新品种保护提供技术支撑。【方法】以柑橘属8个主要栽培种类为试材进行PCR扩增,扩增产物经琼脂糖凝胶电泳检测,筛选出多态性丰富、扩增条带稳定的引物对部分参试品种进行PCR扩增,扩增产物通过变性聚丙烯酰胺凝胶电泳进行进一步的引物筛选;筛选得到的引物进行荧光标记,并选用部分柑橘品种进行PCR扩增,扩增产物利用基因分析仪检测,分别计算各引物的PIC值、等位基因数目,选取一套多态性丰富的适合进行荧光毛细管电泳检测的SSR引物组合,进而对所有参试品种进行分析,构建柑橘品种的DNA指纹图谱库,同时...

【Objective】The purpose of the present work aims at the construction of DNA fingerprint library of citrus cultivar by using a series of SSR core primers in order to provide references for citrus nursery plant identification, cultivars registration and plant variety protection. 【Method】SSR primers were primarily screened by analyzing 8 mainly cultivated species belonging to Citrus while the PCR products were separated by agarose gel electrophoresis, the primers that generated high polymorphic and stable PCR products were chosen for further PC...