期刊论文

Regulation of GT and GATA Transcription Factors on Promoter Function of BnA5.FAD2 and BnC5.FAD2 Genes in Brassica napus

中文

中国农业科学

0578-1752

2018

51

24

4603-4614

10.3864/j.issn.0578-1752.2018.24.002

国家重点基础研究发展计划“973”计划（2015CB150200）；

本校为第一机构

【目的】脂肪酸去饱和酶2基因(FAD2)是控制油菜中油酸含量的重要基因,通过研究GATA和GT转录因子与BnA5.FAD2和BnC5.FAD2启动子的互作关系及其转录调控机制,为高油酸油菜育种提供分子理论基础。【方法】通过缺失启动子片段及生物信息学分析,预测BnA5.FAD2和BnC5.FAD2启动子区潜在顺式作用元件;从PlantTFDB转录因子数据库中筛选候选转录因子,通过实时荧光定量PCR(qRT-PCR)检测转录因子的表达规律,并与BnA5.FAD2和BnC5.FAD2表达规律对比,进一步筛选候选转录因子;利用酵母单杂交验证转录因子与启动子序列的互作情况;将转录因子与BnA5.FAD2和BnC5.FAD2启动子序列共转入拟南芥,通过Western blot检测报...

【Objective】Fatty acid desaturase 2 gene (FAD2) is an important gene for controlling oleic acid content in rapeseed. And in this paper, the interaction between GATA family and BnA5.FAD2 and BnC5.FAD2 gene promoters or GT family transcription factors and BnA5.FAD2 and BnC5.FAD2 gene promoters was studied to make sure the transcription regulation mechanism to provide the theoretical basis for high oleic acid breeding. 【Method】 The potential cis-elements were predicted by deletion of promoter fragments and bioinformatics analysis, and candidate...