期刊论文

Expression and Structural Analysis of SC MI390-5p and Its Target Genes in Potato Response to Low Temperature

中文

中国农业科学

0578-1752

2019

52

13

2295-2308

10.3864/j.issn.0578-1752.2019.13.009

国家自然科学基金（31371683）；

本校为第一机构

【目的】研究马铃薯富含亮氨酸重复序列(leucine-rich repeat receptor-like protein kinase LRR-RLK)类受体蛋白激酶基因SCLRRK1受miR390(ScmiR390-5p)调控响应非生物胁迫的机理。【方法】通过低温响应马铃薯miRNA测序分析与靶基因预测,发现ScmiR390-5p通过调控1个可能的LRR类受体蛋白激酶基因对低温做出响应;利用实时荧光定量PCR技术(Quantitative Real-time PCR,RT-qPCR)验证ScmiR390-5p和SCLRRK1响应低温胁迫的表达情况;利用RLM-5'RACE确定ScmiR390-5p作用于SCLRRK1的切割位点;利用PCR技术克隆得到SCLRRK1的DNA序列和CDS序列,生物信息学分析SCLRRK1的结构和功能;利用RT-qPCR分析ScmiR390-5p...

The study was carried out to investigate the mechanism of potato leucine-rich repeat receptor-like protein kinase (LRR-RLK) receptor protein kinase SCLRRK1 regulated by miR390 (ScmiR390-5p) in response to abiotic stress.【Method】 By sequencing and analyzing potato miRNAs in response to low temperature conditions and predicting target genes, we found that ScmiR390-5p responded to low temperatures by regulating a potential LRR-like receptor protein kinase gene. The expression levels of ScmiR390-5p and SCLRRK1 in response to low temperature stres...