期刊论文

Zhang, H.

中文

中国农业科学

0578-1752

2022

55

14

2837-2849

10.3864/j.issn.0578-1752.2022.14.013

国家自然科学基金联合基金(U21A20253) 国家现代农业产生技术体系(CARS-41-208) 湖南省***青年人才计划(2020)。

本校为第一且通讯机构

【目的】研究黄羽肉鸡种蛋孵化过程中卵黄微生物区系的动态变化和功能,及卵黄微生物对肉鸡胚胎肠道早期微生物定殖的影响。【方法】选取35周龄黄羽肉鸡种蛋100枚,放置在全自动孵化箱中孵化。种蛋入孵前对孵化箱进行清洁消毒,检查内部通风系统和湿度控制系统是否正常工作。孵化温度控制在(37.5±1)℃,湿度控制在(60±10)%。在种蛋孵化的第7、11、15和19天随机选取6枚种蛋在无菌操作台中剥去蛋壳及尿囊膜,使胚胎露出卵黄囊后通过无菌注射器刺入,收集卵黄样品。在孵化的第19和21天在超净工作台中使用经灭菌的手术刀和镊子解剖鸡胚,切开鸡胚腹部后收集胚胎中全肠道样品。样品采集后迅速放入液氮中速...

[Objective] The purpose of this experiment was to study the dynamic changes and functions of the yolk microbiota during the hatching of yellow-feathered broiler fertilized eggs, and the influence of yolk microbiota on the early intestinal colonization of embryos. [Method] One hundred fertilized eggs of 35-week-old yellow-feathered broilers were selected and placed in automatic incubators for incubation. Before hatching, the incubator was cleaned and disinfected, and the internal ventilation system and humidity control system were checked for no...