Starch nanoparticles (SNPs) have received considerable attention due to their biodegradability, nontoxicity, and tunable functional properties. In this study, SNPs were prepared by treating four different botanical origins of starch (wheat, rice, cassava, and potato) with a deep eutectic solvent (DES) consisting of choline chloride and oxalic acid dihydrate. Subsequently, the microstructure and physicochemical properties of SNPs prepared from starch of different botanical origins were systematically investigated. The relative crystallinity of SNPs was significantly lower than that of native st...