期刊论文

Cai, Changqun

英文

Talanta

0039-9140

2018

186

473-480

10.1016/j.talanta.2018.04.060

National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [21775132, 21505112]; Scientific Research Foundation of Hunan Provincial Education DepartmentHunan Provincial Education Department [16A204]; National Natural Science Foundation of Hunan provinceNatural Science Foundation of Hunan Province [2018JJ2388]; Hunan 2011 Collaborative Innovation Center of Chemical Engineering & Technology with Environmental Benignity and Effective Resource Utilization; "1515" academic leader team program of Hunan Agricultural University

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In this study, an intelligent and label-free sensor is utilized for the first time to one-spot simultaneous detection hepatocellular carcinoma markers AFP and miRNA-122 by a resonance light scattering (RLS) sensor. cDNA1 hybridizes with cDNA2 to form double-stranded DNA (dsDNA). The construction of dsDNA and methyl violet is used to form the RLS sensor via the electronic interaction. When AFP or miRNA-122 is present, the cDNA (cDNA1 or cDNA2) can bindings of target, thereby RLS intensity changed proportionally with the concentration of AFP or that of miRNA-122. The detection limits of AFP and ...