期刊论文

中文

棉花学报

1002-7807

2017

29

3

222-232

10.11963/1002-7807.cpyzj.20170322

2014ZX0800501B-003:国家科技重大专项 U1403284:国家自然科学基金 ZR2014CQ047:山东省自然科学基金 NO.ts201511070:山东省"泰山学者"建设工程专项

本校为第一机构

【目的】KFB(Kelch containing F-box protein)是普遍存在于各种生物体中含有F-box结构域的一类家族蛋白,参与众多生物过程。本研究旨在开展陆地棉KFB家族基因鉴定和表达分析。【方法】利用生物信息学方法从陆地棉(TM-1)基因组中鉴定KFB家族成员,与已知的拟南芥、水稻、大豆等KFB蛋白序列构建系统进化树;进一步针对第Ⅱ亚族进行基因结构、染色体定位和表达分析。【结果】从陆地棉基因组共鉴定出150个KFB家族成员,进化分析表明这些基因可分为7个亚族。陆地棉KFB第Ⅱ亚族24个基因分布在14条染色体上,有9对直系同源基因;该亚族基因结构简单,半数基因只有1个外显子;qRT-PCR结果表明,该亚族基因具有较...

[Objective] The KFB （Kelch containing F-box protein） family, involving in many physiological pathways, is a subfami- ly of the biggest F-box protein family in all organisms. In the study, we aims to identify the KFB family members in the upland cotton genome and analyze their expression patterns. [Method] The KFB genes were identified from the whole genome of upland cotton （Gossypium hirsutum L. acc. TM-1） by bioinformatics methods. A phylogenetic analysis was conducted based on the alignment of the KFB proteins from upland cotton, Arabidop...