期刊论文

Genome-Wide Identification and Functional Analysis of theNF-YA Gene Family in Gossypium hirsutum L

中文

棉花学报

1002-7807

2020

32

04

316-328

10.11963/1002-7807.palz.20200611

国家现代农业产业技术体系棉花体系（CARS-15-05） 国家自然科学基金（31671742和31601345） 泰山学者计划（No.ts201511070） 山东省农科院创新工程（CXGC2016A01）

本校为第一机构

【目的】通过对陆地棉NF-YA基因家族进行全基因组鉴定,分析其表达特性,鉴定其中与棉花开花相关的基因。【方法】利用生物信息学方法系统分析了其理化性质、基因结构、共线性、Ka/Ks、顺式作用元件和表达模式,实时荧光定量聚合酶链反应(Polymerase chain reaction,PCR)分析表达特征,采用病毒诱导基因沉默技术验证基因功能。【结果】陆地棉中鉴定到29个NF-YA基因家族成员,分为5个亚组,定位在18条染色体上;全基因组复制和片段复制是GhNF-YA基因家族扩张的主要动力;启动子区含有大量光反应的顺式作用元件。GhNF-YA基因家族基因在茎、叶中高表达;12个GhNF-YA基因在早熟品种鲁棉研19号和晚熟品种鲁棉研...

[Objective] The NF-YA gene family was identified in upland cotton (Gossypium hirsutum L.), and their expression characteristics were analyzed to discover the genes related to cotton flowering regulation. [Method] Using bioinformatic methods, the NF-YA genes were identified and their physical and chemical properties, gene structure, cis-element, collinearity, Ka/Ks were investigated. The expression pattern was performed using quantitative real-time polymerase chain reaction (qRT-PCR). The gene function was verified by virus-induced gene silencing (VIGS). [Result] A total of 29 GhNF-YA genes wer...