[Objective]The acyl-coenzyme A oxidase(ACX)gene family was identified in Gossypium hirsutum and their expression patterns were analyzed,which would lay the foundation for further studies of the function of ACX genes.[Method]The members of ACX gene family in G.hirsutum genome were identified by bioinformatics method,and their physical and chemical properties,gene structure,evolutionary relationships,gene replication,cis-acting elements in promoter region and expression patterns were analyzed systematically,and the virus-induced gene silencing(VIGS)technique was used to preliminary explore the f...