Hunan Provincial Natural Science FoundationNatural Science Foundation of Hunan Province [2017JJ3221]; National Science FoundationNational Science Foundation (NSF) [31370271]
机构署名:
本校为第一机构
院系归属:
生物科学技术学院
摘要:
RNA editing in chloroplasts and mitochondria is performed by hypothetical editosomes. The MORF family proteins are essential components of these editosomes. In Arabidopsis, MORF2 and MORF9 are involved in the editing of most sites in chloroplasts. In this work, we performed immunoprecipitation and mass spectrometry assays of transgenic lines expressing MORF2-4xMYC and MORF9-4xMYC to identify interacting proteins. We found that MORF2 and MORF9 are present in the same complex. Blue-Native PAGE analysis of chloroplast protein complexes also revealed that both MORF2 and MORF9 are part of a complex...