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MORF2 tightly associates with MORF9 to regulate chloroplast RNA editing in Arabidopsis

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成果类型:
期刊论文
作者:
Huang, Chao;Li, Zi-Ran;Yu, Qing-Bo;Ye, Lin-Shan;Cui, Yong-Lan;...
通讯作者:
Yang, Zhong-Nan
作者机构:
[Huang, Chao] Hunan Agr Univ, Hunan Prov Key Lab Phytohonnones & Growth Dev, Changsha 410128, Hunan, Peoples R China.
[Yang, Zhong-Nan; Ye, Lin-Shan; Yu, Qing-Bo; Li, Zi-Ran; Cui, Yong-Lan] Shanghai Normal Univ, Coll Life Sci, Shanghai 200234, Peoples R China.
[Molloy, David P.] Hunan Univ Arts & Sci, Int Coll, Changde 415000, Peoples R China.
通讯机构:
[Yang, Zhong-Nan] S
Shanghai Normal Univ, Coll Life Sci, Shanghai 200234, Peoples R China.
语种:
英文
关键词:
MORFs;Editosome;chloroplast;Arabidopsis
期刊:
Plant Science
ISSN:
0168-9452
年:
2019
卷:
278
页码:
64-69
基金类别:
Hunan Provincial Natural Science FoundationNatural Science Foundation of Hunan Province [2017JJ3221]; National Science FoundationNational Science Foundation (NSF) [31370271]
机构署名:
本校为第一机构
院系归属:
生物科学技术学院
摘要:
RNA editing in chloroplasts and mitochondria is performed by hypothetical editosomes. The MORF family proteins are essential components of these editosomes. In Arabidopsis, MORF2 and MORF9 are involved in the editing of most sites in chloroplasts. In this work, we performed immunoprecipitation and mass spectrometry assays of transgenic lines expressing MORF2-4xMYC and MORF9-4xMYC to identify interacting proteins. We found that MORF2 and MORF9 are present in the same complex. Blue-Native PAGE analysis of chloroplast protein complexes also revealed that both MORF2 and MORF9 are part of a complex...

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