期刊论文

Expression of 5 fatty acid metabolism-related genes in different growth rapeseed stages

中文

生物学杂志

2095-1736

2016

33

1

32-34,48

10.3969/j.issn.2095-1736.2016.01.032

31201240:国家自然科学基金 31000722:国家自然科学基金 2015CB150206:国家重点基础研究发展计划（973计划） 2012AA101107－3:国家高技术研究发展计划（863计划） 2014FJ1006:湖南省科技重大专项 2014008:实验室开放基金 ZWKF201302:湖南农业大学作物学开放基金

本校为第一机构

为了明晰与脂肪酸代谢相关基因在不同油酸含量油菜中的表达情况,以出苗后7 d、5 ~6叶期和越冬期的叶片、花瓣和授粉后20 ~ 35 d的种子为材料,对5个转录组分析得到的差异基因(Bna0691280,Bna0421530,Bna0547440,Bna0799930和Bna0764010)在高油酸油菜近等基因系中的表达情况进行研究。结果发现,在营养生长期(出苗后7 d、5~6叶期和越冬期)的叶片中,5个基因在高油酸油菜材料中的表达量均高于低油酸油菜中的表达量。在花中除Bna0547440和Bna0764010外,其它3个基因在高油酸油菜中的表达量高于低油酸油菜中的表达量。在授粉后20 ~35 d的种子中除Bna0799930外,其余4个基因在高油酸油菜材料中的表达量均低...

In order to understand the expression of fatty acid metabolism-related genes in rapeseed materials with different oleic acid contents, in this study, the leaves of 7-day seedlings, in 5-6 leaf stage and overwintering period , petals and the seeds of 20-35 days after pollination were used as materials , and the expression of 5 differential genes ( Bna0691280, Bna0421530, Bna0547440, Bna0799930 and Bna0764010) which obtained by transcriptome analysis of high oleic rapeseed near-isogenic lines were studied .The results showed that:in the leaves , the expression levels in high oleic rapeseed mater...