期刊论文

中文

生物学杂志

2095-1736

2020

37

3

21-25

10.3969/j.issn.2095-1736.2020.03.021

2018JJ3380:湖南省自然科学基金 2017JC08:湖南省农科院科技创新项目

本校为第一机构

Pik位点是水稻第11染色体上稻瘟病抗性基因成簇分布的热点区域,对该位点抗性基因在水稻品种中多样性分布的研究,有助于揭示该基因的进化规律和进一步挖掘其等位基因。对来自不同国家和地区的400份非洲稻和400份亚洲稻稻种抗稻瘟病基因Pik-2编码区序列进行了测序分析,发现非洲稻和亚洲稻品种Pik-2编码区碱基变异非常丰富,非洲稻种中出现了113个变异位点,亚洲稻种中有78个碱基位点发生多样性变异,其中两者均在1879位点显示出极高的变异频率;根据其碱基变异特点,非洲稻种和亚洲稻种分别呈现出17和12种单倍型。单倍型的地区分布研究显示,非洲稻的分布呈现出大聚集、小分散的特点,而亚洲稻的分布来源...

The Pik locus, located on the long arm of chromosome 11, is a hotspot region for rice blast resistance. To study the diversity and distribution of the resistance(R) genes at the Pik locus will be useful for revealing the evolutionary mechanisms of this gene and to further explore its alleles. We sequenced and analyzed the Pik-2 coding region of 400 Oryza glaberrima and 400 Oryza sativa cultivars from different countries and regions. 113 and 78 polymorphic sites were found in O. glaberrima and O. sativa, respectively, which showed high mutation frequency at the nucleotide position 1879. Accordi...