期刊论文

High-throughput analysis of bacterial diversity in highland barley cultivation soil in Lhasa

中文

生物学杂志

2095-1736

2020

37

2

46-51

10.3969/j.issn.2095-1736.2020.02.046

XZNKY-2018-C-026:西藏自治区财政专项 XZ2017ZRG-42:西藏自治区自然科学基金 kq1706025:长沙市科技计划 SYL201802002:湖南农业大学双一流建设项目

本校为第一机构

为了解拉萨地区青稞种植农田土壤中细菌群落结构,明确不同青稞生长时期和不同地点对细菌多样性的影响,以青稞种植土壤为试验材料,通过高通量测序技术分析青稞种植土壤中细菌的群落结构及多样性。结果显示:本次种植青稞土壤微生物测序每个样品获得37 265 ~ 40 008条有效序列和4094 ~ 4730个OTU,稀释曲线和测序深度指数反映此次测序结果比较全面地覆盖了土壤微生物群落;不同时期各样品的Shannon指数和OTU数量均值大小为Group 1(3月25日、播种前期)< Group 2(7月8日、灌浆期)< Group 3(9月28日、收获后期),不同时期菌落组成和菌落数量在Gp16、Gaiella、Ilumatobacter和Nocardioides等存在差异;随着...

In order to understand the bacterial community structure in the farmland soil of highland barley planting in Lhasa,and to clarify the influence of different growing periods and different locations of highland barley on the bacterial diversity,the community structure and diversity of bacteria in highland barley cultivation soil were analyzed by high-throughput sequencing technology with the highland barley cultivation soil as the experimental material. Result showed that 37 265-40 008 effective sequences and 4094-4730 OTU were obtained from each sample of highland barley soil microbial sequenci...