期刊论文

中文

生物学杂志

2095-1736

2022

39

2

69-73,79

10.3969/j.issn.2095-1736.2022.02.069

湖南省科技重大专项（2018NK1030）； 湖南省科技特派员创新创业项目（2020NK4194）； 湖南战略性新兴产业科技攻关与重大科技成果转化项目（2019GK4018）； 湖南农业大学“双一流”建设项目（SYL201802002）；

本校为第一机构

为筛选获得稳定高效的自生固氮菌，从多个地区植物根际土壤以及淤泥中选取样品，以无氮培养基进行分离筛选，采用乙炔还原法测定固氮酶活性，经形态学观察、生理生化试验以及16S rDNA序列分子鉴定，并通过单因素与响应面试验相结合的方式对生长条件进行优化。结果表明：从山西省朔州市玉米根际的土壤中筛选到一株固氮酶活性达140.14 nmol C2H4/mg.protein.hr的自生固氮菌，该菌鉴定为Azotobacter属，命名Azotobacter sp. strain C-5-2；菌株最适生长的条件为蔗糖浓度10 g/L、转速148 r/min、温度32℃以及初始pH 9.0。

In order to screen and obtain stable and efficient abiogenousazotobacter,samples were selected from the soil of plant rhizosphere and silt in multiple regions,separated and screened with nitrogen-free medium,and nitrogenase activity was determined by acetylene reduction method.Morphological observation,physiological and biochemical test and 16S rDNA sequence molecular identification were made.Furthermore,the growth conditions were optimized through a combination of single factor and response surface test.The results showed that a strain of abio...