期刊论文

Li, CX

中文

化学学报

0567-7351

2003

61

5

790-794

10.3321/j.issn:0567-7351.2003.05.026

国家自然科学基金 （Nos.2 0 0 750 0 6 ,2 99750 0 6）； 湖南省自然科学基金 （Nos.0 1JJY1 0 0 3 ,0 1JJY2 0 99）； 博士点基金 （No.2 0 0 1 0 532 0 0 8）；

本校为其他机构

提出了一种酶标识抗原与待测样品的竞争免疫反应定量测定IAA的方法．该方法是基于金基底上形成了均一、稳定、有序的硫基自组装单层膜，能以共价方式固定抗体．利用循环伏安法探讨了底物在不同条件下的反应特征，并研究了最佳实验条件．在浓度5．68×10^-7～2.83×10^-5mol/L范围内、-200mV电位下，响应电流与lg[IAA]呈线性关系，其回归方程；i(nA)=-20.8333×lg(10^6[IAA])+68.9167，相关系数0.9915。对样品IAA的含量进行测定，结果令人满意．

A competitive binding assay used to determine hapten IAA with the aid of HRP-IAA conjugate has been developed. It is based on the ordered, stable mercapto self-assembled monolayer formed on gold surface, which immobilized IgG with covalent bond. The features of the substrate reaction under different conditions were investigated by cyclic voltammetry and the assay conditions were optimized. Under applied potential of -200 mV, the response current is linear with Ig[IAA], over a concentration range of 5.68 × 10-7 - 2.83 × 10-5 mol/L with a corre...